QIAseq FastSelect: Increase your unique reads, discover more biology

The emerging importance of rRNA removal in RNA-seq optimization

Did you know that a critical step for RNA-seq optimization is efficient removal of rRNA? The amount of rRNA removed is directly proportional to the number of unique gene reads obtained from your library. Efficient, near complete rRNA removal prior to sample cDNA synthesis ensures you aren't making unwanted ribosomal cDNA. Introduce our game-changing QIAseq FastSelect technology into your workflow and experience highly efficient rRNA removal in as little as 14 minutes. In fact, samples treated with QIAseq FastSelect don't require pre-sequencing PCR amplification – simply use your library as is, save even more time and obtain a higher number of unique gene reads. FastSelect your sample before library prep and discover more biology.

New! QIAseq FastSelect –5S/16S/23S Kits

Highly abundant bacterial 5S,16S and 23S rRNA wastes precious sequencing resources and reduces the number of unique reads you obtain from your RNA-seq library. New QIAseq FastSelect –5S/16S/23S Kits are a revolutionary solution for pan-bacterial 5S,16S and 23S rRNA removal from fragmented or full-length RNA for metatranscriptomics studies. No need for capture or enzymatic digestion. Simply use our 14-minute protocol, followed by bead cleanup for removal of unwanted rRNA from complex bacterial samples.

QIAseq FastSelect –rRNA HMR and QIAseq FastSelect –Globin Kits

Performing RNA-seq with mammalian tissue, organ or liquid biopsy samples? Our QIAseq FastSelect –rRNA HMR and QIAseq FastSelect –Globin Kits are single kit solutions that allow you to remove >95% of rRNA and globin mRNA from human, mouse, rat and other mammalian samples. With a 30% faster protocol than our previous kit versions, QIAseq FastSelect enables RNA removal using just a single 10-second pipetting step, with only 14 minutes of incubation.

QIAseq FastSelect success stories: Inspiration from the lab bench

QIAseq FastSelect helps pave the way towards more successful cancer treatment

“QIAseq FastSelect has been phenomenal with the RNA sequencing in my project. The RNA was degraded and almost unusable, but QIAseq FastSelect really removes the ribosomal RNA in these degraded samples and has improved our sequencing libraries.”

Brandon Mistretta, University of Houston’s Department of Biology and Biochemistry

Cancer researchers at the University of Houston routinely encountered challenges associated with RNA degradation when working with FFPE samples. The presence of unwanted RNA species such as ribosomal RNA and globin mRNA in the low-yield RNA sample further complicated RNA sequencing. Read how QIAseq FastSelect transformed their research, allowing them to retrieve useful RNA-seq data from FFPE RNA.

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QIAseq FastSelect enables construction of RNA-seq libraries from low-quality FFPE RNA

“The workflow is very simple and fast. I would say that with poor-quality samples and with the limited quantity of those samples, I wasn’t expecting the results we obtained.”

Dr. Fabienne Desmots-Loyer, Hematology researcher, CHU – Hospital Pontchaillou, Rennes, France

Due to high levels of degradation and poor yields, working with RNA from FFPE samples can be tough. Read the story of Dr. Fabienne Desmots-Loyer at the Hospital Rennes and find out how QIAseq FastSelect saved the day by providing high-quality libraries from FFPE cancer samples previously rejected for processing by two commercial labs due to high levels of RNA degradation and low yields.

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One small step in RNA-seq, one giant leap for your research

While alternative rRNA removal methods take several hours, require multiple steps, work solely on unfragmented RNA or simply don’t remove enough rRNA, with the FastSelect method you benefit from a dramatically faster and more convenient workflow – and exceptional results even with FFPE and fragmented RNA samples.

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