Did you know that a critical step for RNA-seq optimization is efficient removal of rRNA? The amount of rRNA removed is directly proportional to the number of unique gene reads obtained from your library. Efficient, near complete rRNA removal prior to sample cDNA synthesis ensures you aren't making unwanted ribosomal cDNA. Introduce our game-changing QIAseq FastSelect technology into your workflow and experience highly efficient rRNA removal in as little as 14 minutes. In fact, samples treated with QIAseq FastSelect don't require pre-sequencing PCR amplification – simply use your library as is, save even more time and obtain a higher number of unique gene reads. FastSelect your sample before library prep and discover more biology.
Working with mixed human and microbiome samples from nasopharyngeal, lung and other tissues for coronavirus metatranscripomics studies? To maximize unique mRNA/gene expression reads, use QIAseq FastSelect –rRNA HMR Kits and QIAseq FastSelect –5S/16S/23S Kits separately or in combination for fast, effective and targeted removal of both host and bacterial rRNA – while still retaining viral RNAs.
“QIAseq FastSelect has been phenomenal with the RNA sequencing in my project. The RNA was degraded and almost unusable, but QIAseq FastSelect really removes the ribosomal RNA in these degraded samples and has improved our sequencing libraries.”
Brandon Mistretta, University of Houston’s Department of Biology and Biochemistry
Cancer researchers at the University of Houston routinely encountered challenges associated with RNA degradation when working with FFPE samples. The presence of unwanted RNA species such as ribosomal RNA and globin mRNA in the low-yield RNA sample further complicated RNA sequencing. Read how QIAseq FastSelect transformed their research, allowing them to retrieve useful RNA-seq data from FFPE RNA.
“The workflow is very simple and fast. I would say that with poor-quality samples and with the limited quantity of those samples, I wasn’t expecting the results we obtained.”
Dr. Fabienne Desmots-Loyer, Hematology researcher, CHU – Hospital Pontchaillou, Rennes, France
Due to high levels of degradation and poor yields, working with RNA from FFPE samples can be tough. Read the story of Dr. Fabienne Desmots-Loyer at the Hospital Rennes and find out how QIAseq FastSelect saved the day by providing high-quality libraries from FFPE cancer samples previously rejected for processing by two commercial labs due to high levels of RNA degradation and low yields.
While alternative rRNA removal methods take several hours, require multiple steps, work solely on unfragmented RNA or simply don’t remove enough rRNA, with the FastSelect method you benefit from a dramatically faster and more convenient workflow – and exceptional results even with FFPE and fragmented RNA samples.
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