Targeted whole viral genome library preparation

RNA viruses such as the novel Coronavirus (SARS-CoV-2) are known to accumulate genomic variations that may result in changes in virulence, infectivity and transmissibility (1). Tracking genomic epidemiology and monitoring rapid viral evolution is essential for improved understanding of the virus (2). The ARTIC Network has proposed direct amplification of the virus using tiled, multiplex primers and has been widely adopted by groups worldwide based on a published reference SARS-CoV-2 genome MN908947.3.

The benefits of using tiled amplicon sequencing are:

  • Detection of very low viral genome copy numbers
  • Faster workflow compared to alternatives such as hybrid capture, which can take up to 16 hours
  • Compatible with degraded RNA samples
  • Reduction of downstream sequencing costs

QIAGEN is in the process of developing the QIAseq SARS-CoV-2 Primer Panel, a multiplex PCR primer set for whole genome amplification of the novel Coronavirus, SARS-CoV-2. Based on the ARTIC V3 primers described here, the QIAseq SARS-CoV-2 Primer Panel separates 400 amplicons into two PCR pools that together cover the entire SARS-CoV-2 genome.

With every purchase of our QIAseq FX DNA Library Kit, you could be among the first group of researchers to get early access to our new QIAseq SARS-CoV-2 Primer Panel.

References

  1. Shen, Z. et al. (2020) Genomic diversity of SARS-CoV-2 in Coronavirus Disease 2019 patients. Clin Infect Dis. ciaa203. doi: 10.1093/cid/ciaa203. [Epub ahead of print]
  2. Phan, T. (2020) Genetic diversity and evolution of SARS-CoV-2. 81:104260. doi: 10.1016/j.meegid.2020.104260. [Epub ahead of print]

Please submit this form to request an early-access kit

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Free temporary licenses are currently available for COVID-19 research.





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